in-vitro cellular uptake and transport study of 9-nitrocamptothecin plga nanoparticles across caco-2 cell monolayer model
نویسندگان
چکیده
the uptake and transport of 9-nitrocamptothecin (9-nc), a potent anticancer agent, across caco-2 cell monolayers was studied as a free and plga nanoparticle loaded drug. different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. the transport of nanoparticles across the caco-2 cell monolayer as a function of incubation time and concentration was evaluated for each different nanoparticle formulation. the amount of 9-nc transported from the apical to the basolateral side and the uptake of the drug into the cells was determined by hplc. the uptake of intact nanoparticles into caco-2 cells was visualized by confocal laser scanning microscopy using 6-coumarin as a fluorescent marker. the study demonstrated that caco-2 cell uptake and transport of encapsulated 9-nitrocamptothecin is significantly affected by the diameter of the carrier and incubation time. in addition it was shown to be independent of concentration. the results indicated a significant accumulation of the drug in the cell membrane and an enhanced diffusion across the cell membrane. there was also a sustained release of characteristics pertaining to polymeric carriers that provided prolonged drug availability for absorptive cells.
منابع مشابه
In-vitro cellular uptake and transport study of 9-nitrocamptothecin PLGA nanoparticles across Caco-2 cell monolayer model
The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...
متن کاملIn-vitro cellular uptake and transport study of 9-nitrocamptothecin PLGA nanoparticles across Caco-2 cell monolayer model
The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...
متن کاملIn-vitro Cellular Uptake and Transport Study of 9-Nitrocamptothecin PLGA Nanoparticles Across Caco-2 Cell Monolayer Model
The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...
متن کاملTransport Across Caco-2 Monolayer: Biological, Pharmaceutical and Analytical Considerations
متن کامل
study of the copolymer structure effect on physicochemical characteristics and in vitro stability of plga–peg nanoparticles loaded 9-nitrocamptothecin
9-nitrocamptothecin (9-nc) is a semisynthetic and a low soluble analogue of camptothecin alkaloids that target nuclear enzyme topoisomerase i. the unstable lactone form of 9-nc in biological fluids requires for its cytotoxic activity. to improve aqueous solubility and stability in biological media, 9-nc was loaded in polymeric nanoparticles. in this paper, we studied the effect of peg percent (...
متن کاملBiodegradable nanoparticles containing protein-fatty acid complexes for oral delivery of salmon calcitonin.
Biodegradable nanoparticles containing salmon calcitonin (sCT) were formulated using protein-fatty acid complexes, and their in vitro transport against a Caco-2 cell monolayer and the extent of in vivo oral uptake were assessed. Positively charged sCT was hydrophobically ion paired to form physical complexes with fatty acid, phospholipid, and surfactant. Among the complexes, sodium oleate was u...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of pharmaceutical researchجلد ۲۰۱۱، شماره ۹، صفحات ۴۲۵-۴۳۴
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023